山羊Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

概述

  • 产品名称
    山羊Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG - H&L (Cy3 ®), pre-adsorbed
  • 靶标种属
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物
    Cy3 ®. Ex: 552nm, Em: 565nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • 共轭说明
    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/100. PubMed: 17045908
ICC Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed 图像

  • Ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)参考文献

This product has been referenced in:
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). ICC/IF . Read more (PubMed: 28158775) »
  • Gruntenko NE  et al. The impact of FOXO on dopamine and octopamine metabolism in Drosophila under normal and heat stress conditions. Biol Open 5:1706-1711 (2016). IHC . Read more (PubMed: 27754851) »

See all 23 Publications for this product

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