山羊Anti-Rabbit IgG H&L (Biotin) (ab97049)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG - H&L (Biotin)
  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • 共轭说明Biotin/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. ELISA-Primary
WB 1/20000 - 1/400000. WB-Chemiluminescent

Goat Anti-Rabbit IgG H&L (Biotin) 图像

  • Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

    Image on the left: isotype control image

    This image is courtesy of Dr Kyle Caires

Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)参考文献

This product has been referenced in:
  • Vyazunova I  et al. Sleeping Beauty mouse models identify candidate genes involved in gliomagenesis. PLoS One 9:e113489 (2014). IHC-P . Read more (PubMed: 25423036) »
  • Santos Nassif Lacerda SM  et al. Phenotypic characterization and in vitro propagation and transplantation of the Nile tilapia (Oreochromis niloticus) spermatogonial stem cells. Gen Comp Endocrinol 192:95-106 (2013). Read more (PubMed: 23792279) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ki-67 staining in mouse prostate tissue using Abcam secondary antibody Goat anti-Rabbit IgG H&L (Biotin) (ab97049)
Username

Abcam user community

Verified customer

提交于 Apr 08 2015

Thank you for contacting us and you interest in our products.

There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of...

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Thank you so much for your call today and for letting us know about the trouble with the staining.

As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***. ...

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Merci de nous avoir contactés.

Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269

...

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Thank you for contacting Abcam.

Please find attached the requested Certificate of Compliances.

If there is anything else I can help you, please let me know.

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.
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I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

Thank you for your email! The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"