山羊Anti-Rabbit IgG H&L (AMCA) (ab123435)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (AMCA)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: ICC/IF, WB, Flow Cytmore details
  • 偶联物AMCA. Ex: 350nm, Em: 445nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.20
    Preservative: 0.013% Sodium azide
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 共轭说明Blue fluorescence. Excitation/Emission wavelength= 344 nm/ 446 nm
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab123435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 - 30 µg/ml.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (AMCA) 图像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained stained with DRAQ7TM (ab109202)

Goat Anti-Rabbit IgG H&L (AMCA) (ab123435)参考文献

ab123435 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"