山羊抗兔IgG H&L (ab2892)
Key features and details
- Goat Anti-Rabbit IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ICC
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
This antibody cocktail reacts with heavy and light chains on rabbit IgG and mouse IgG (reacts with all IgG subtypes). -
经测试应用
适用于: IHC-P, IHC-Fr, ICCmore details
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.04% Proclin 300, 0.5% Proclin 950, 99% Water -
Concentration information loading...
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共轭说明
HRP or AP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The end result is two antibody subsets: 1) Multiple HRP molecules linked to goat anti-rabbit antibodies. 2) Multiple AP molecules linked to goat anti-mouse antibodies. -
克隆
多克隆 -
同种型
IgG -
常规说明
This product is specially designed for a rapid double stain procedure. The overall staining procedure can be done in 4 steps and in less than two hours. Avidin-biotin blocking procedures are not necessary when using this product. This new and innovative AP and HRP polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP or HRP conjugated secondary antibodies.
This product is provided in a pre-diluted, ready-to-use format. Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS. Equally suited to both manual and automated immunostaining proceedures. This product has not been tested in any application requiring further dilution of the antibody and therefore it is not recommended for use in such applications.
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研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/1.
|
|
IHC-Fr |
1/1.
|
|
ICC |
1/1.
|
说明 |
---|
IHC-P
1/1. |
IHC-Fr
1/1. |
ICC
1/1. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab2892 尚未被引用在任何文献中。