The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed (ab27237)Image from Berger M et al, J Biol Chem. 2012 Jun 1;287(23):19409-17. Epub 2012 Apr 24, Fig 3. DOI 10.1074/jbc.M112.353557 June 1, 2012 The Journal of Biological Chemistry, 287, 19409-19417.
ab27237 used in Electron Microscopy. Subcellular localization of RIG-I in human neutrophils. C: Isolated peripheral blood neutrophils were fixed, washed with PBS, permeabilized, and blocked (10% normal goat serum, 10 mm glycine, and 0.2% Tween 20 in diluent containing 0.5% bovine serum albumin and 0.5% Triton X-100 in PBS). For visualization of RIG-I, the grids were incubated with anti-RIG-I antibody and a gold-conjugated secondary antibody (gold sphere diameter, 5 nm; ab27237, Abcam). Finally, the grids were stained with 1% uranyl acetate. Negative controls were obtained by omitting the primary antibody. Arrows indicate RIG-I staining. The red arrows mark RIG-I associated with a vesicle-like structure.
This product has been referenced in:
Marcos V et al. Free DNA in cystic fibrosis airway fluids correlates with airflow obstruction. Mediators Inflamm2015:408935 (2015).
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