山羊Anti-Rabbit IgG H&L (20nm Gold) preadsorbed (ab27237)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (20nm Gold) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: Electron Microscopymore details
  • 最小交叉反应
    Human more details
  • 免疫原

    Full length native Rabbit IgG

  • 偶联物Gold 20nm

性能

应用

Our Abpromise guarantee covers the use of ab27237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy
  • 应用说明Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed 图像

    • ab27237 used in Electron Microscopy.
      Subcellular localization of RIG-I in human neutrophils.

      C: Isolated peripheral blood neutrophils were fixed, washed with PBS, permeabilized, and blocked (10% normal goat serum, 10 mm glycine, and 0.2% Tween 20 in diluent containing 0.5% bovine serum albumin and 0.5% Triton X-100 in PBS). For visualization of RIG-I, the grids were incubated with anti-RIG-I antibody and a gold-conjugated secondary antibody (gold sphere diameter, 5 nm; ab27237, Abcam). Finally, the grids were stained with 1% uranyl acetate. Negative controls were obtained by omitting the primary antibody.

      Arrows indicate RIG-I staining. The red arrows mark RIG-I associated with a vesicle-like structure.

    Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed (ab27237)参考文献

    This product has been referenced in:
    • Berger M  et al. Neutrophils Express Distinct RNA Receptors in a Non-canonical Way. J Biol Chem 287:19409-17 (2012). Electron Microscopy . Read more (PubMed: 22532562) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab27237.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"