山羊Anti-Mouse IgM mu chain (Agarose) (ab65867)

概述

  • 产品名称山羊Anti-Mouse IgM mu chain (Agarose)
    参阅全部 IgM 二抗
  • 靶标种属Mouse
  • 特异性Specificity for the mu-chain of mouse IgM is determined by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses) and IgM myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to agarose bead coupling. Electrophoresis of the product followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the gamma region.
  • 免疫原

    Mouse IgM

  • 偶联物Agarose

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: 2.9% Sodium chloride
  • Concentration information loading...
  • 纯度IgG fraction
  • 共轭说明The IgG fraction of goat anti-mouse IgM (mu-chain specific) antiserum is covalently attached to cyanogen bromide activated cross-linked beaded agarose. Five to ten milligrams of IgG fraction is bound per milliliter of resin. After equilibration, a minimum of 0.4 mg of mouse IgM can be bound and eluted per milliliter of packed resin.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Procedure
    A two milliliter column of antibody-agarose is prepared using four milliliters of the antibody-agarose suspension. The column is equilibrated in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M NaCl (PB). The antigen solution to be bound is applied slowly and followed by a PB wash. Unbound effluent fractions are collected and assayed for protein content (Lowry). The column is then stripped by washing with 0.1 M glycine, 0.15 M NaCl, pH 2.4, or 0.5 M acetic acid, 0.15 M NaCl, pH 2.4. Fractions containing protein are collected, brought to neutral pH and assayed for protein content (Lowry).

    Regeneration
    Goat Anti-Mouse IgM-Agarose may be regenerated and used for future adsorptions. Strip the agarose with ten column volumes of 0.1 M glycine, 0.15 M sodium chloride, pH 2.4, or 0.5 M acetic acid, 0.15 M sodium chloride, pH 2.4, then wash with 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M sodium chloride (PB).
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent dilution.

Goat Anti-Mouse IgM mu chain (Agarose) (ab65867)参考文献

ab65867 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam technical services regarding ab65867 - Anti-Mouse IgM.   I can confirm that this product has been tested for affinity purification only and we haven't tested it for immunoprecipitation specifically. As the principle i...

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