By immunoelectrophoresis and ELISA this antibody reacts with mouse IgG, IgA and IgM. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat immunoglobulins was detected. This antibody may cross react with immunoglobulins from other species.
Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.