山羊Anti-Mouse IgG2b heavy chain (Biotin) preadsorbed (ab98701)

概述

  • 产品名称山羊Anti-Mouse IgG2b heavy chain (Biotin) preadsorbed
    参阅全部 IgG2b 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: ICC/IF, IHC-P, ELISA, WBmore details
  • 最小交叉反应
    Human, Rat more details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.
IHC-P 1/250 - 1/2500.
ELISA 1/10000 - 1/200000. (Primary ELISA).
WB 1/10000 - 1/200000. (Chemiluminescent).

Goat Anti-Mouse IgG2b heavy chain (Biotin) preadsorbed (ab98701)参考文献

ab98701 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
fix 5 minutes Histofix (Histolab, Sweden)
permeabilization 5 minutes 0.2 % Triton X-100 in PBS
primary antibody: dilution 1:100 anti -mouse IgG2b myosin heavy chain type 1 in 1% goat serum in PBS (BA-F8, Hybridoma Bank) 2 hours at room temperature or overnight +4 C
secondary antibody: dilution 1:500 biotin IgG2b (ab98701) in 1% goat serum in PBS1 hour RT
detection: streptavidin, Alexa Flour 405 conjugate (Molecular Probes S32351) 45 minutes
other primary antibodies: dilutions 1:100 anti mouse IgG1 myosin heavy chain type 2a (SC-71, Hybridoma bank) and 1:500 polyclonal anti-rabbit dystrophin (ab15277, Abcam)
other secondary antibodies: dilutions 1:500 Alexa Flour 488 anti-mouse IgG1 (Molecular probes A21121) and 1:500 Alexa Flour 555 anti-rabbit (Molecular Probes A21429)
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Dr. Satu Koskinen

Verified customer

提交于 Dec 27 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"