山羊Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

概述

  • 产品名称山羊Anti-Mouse IgG2a heavy chain (HRP)
    参阅全部 IgG2a 二抗
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.
  • 经测试应用适用于: IHC-P, ELISA, WB, ICCmore details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明Molar enzyme/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
ICC Use at an assay dependent dilution.

Goat Anti-Mouse IgG2a heavy chain (HRP) 图像

  • IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab75927, 0.5µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)参考文献

This product has been referenced in:
  • Seth A  et al. Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner. PLoS One 10:e0117203 (2015). Read more (PubMed: 25756283) »
  • Wibowo N  et al. Co-administration of non-carrier nanoparticles boosts antigen immune response without requiring protein conjugation. Vaccine N/A:N/A (2014). Read more (PubMed: 24793947) »

See all 6 Publications for this product

Product Wall

Application Western blot
I tested with 3 mouse tumor lysates and 3 different IgGs.

Tumor lysates have been also pre-cleared with protein A/G beads to get rid of most (but not all) IgG.

No primary antibody was used. This secondary antibody was incubated 1:5000 at room temperature for 1 hour. Here are the results:

1. Ab97245 reacts with mouse IgG2a heavy chain strongly
2. Ab97245 reacts with mouse IgG2a light chain slightly, which is the reason I did not give it a 5-star.
3. Ab97245 does not react with mouse IgG1 or human IgG, neither heavy chain or light chain.
Username

Abcam user community

Verified customer

提交于 Oct 02 2015

Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"