山羊Anti-Mouse IgG1 heavy chain (HRP) preadsorbed (ab98693)

概述

  • 产品名称山羊Anti-Mouse IgG1 heavy chain (HRP) preadsorbed
    参阅全部 IgG1 二抗
  • 描述
    山羊多克隆抗体二抗to小鼠IgG1 - heavy chain (HRP), pre-adsorbed
  • 靶标种属Mouse
  • 经测试应用适用于: ICC, IHC-P, ELISA, WBmore details
  • 最小交叉反应
    Human, Rat more details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent

Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed 图像

  • IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed (ab98693)参考文献

This product has been referenced in:
  • Garrett SM  et al. Agonism of the 5-hydroxytryptamine 1F receptor promotes mitochondrial biogenesis and recovery from acute kidney injury. J Pharmacol Exp Ther 350:257-64 (2014). WB . Read more (PubMed: 24849926) »

See 1 Publication for this product

Product Wall

Application Western blot
Myc tagged protein 65kDa probed with Monoclonal Myc antibody and later with Mouse secondary Heavy chain specific HRP. Lane 5 is negative control with no Myc tagged protein. The antibody is excellent and recognized the specific band. I highly recommend this antibody.
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Abcam user community

Verified customer

提交于 Feb 28 2014

Thank you for contacting us.

The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some op...

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Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

http://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


Here is the discount...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us.

Could you provide more details about the problem?

- was the vial broken
- was the liquid all over the pouch
- When the product was ordered. Could you provide Abcam purchase order number? The purcha...

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Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

Thank you for your email.

The ab98693 is conjugated the HRP enzyme and you will need just the substrate in addition. As a substrate, you can for example use the AEC substrate system ab64252 or the DAB substrate Kit ab64238. The DAB and the AE...

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Thank you for your email.

As x is not in the office today, I am answering on his behalf.

I can confirm that you can use AEC or DAB depending on your preference and the counterstain of your slides. Both of these substrates are adapte...

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Thank you for contacting us.
I am sorry to hear that either one or both antibodies are not working as expected.

As we discussed over the phone, the following suggestions will help us to determine if either one of the antibodies or both o...

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Thank you for contacting us.

1. Yes, a substrate is needed.

2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
> IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"