山羊Anti-Mouse IgG - H&L (SureLight® Allophycocyanin), pre-adsorbed (ab99900)

概述

  • 产品名称
    山羊Anti-Mouse IgG - H&L (SureLight® Allophycocyanin), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 经测试应用
    适用于: Flow Cytmore details
  • 最小交叉反应

    Cow, Rabbit more details
  • 偶联物
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm

性能

  • 形式
    Lyophilised:Reconstitute with 1 ml of ddH2O
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: None
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 10mM Tris,1 ug/ml Pentachlorophenol pH 8.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 共轭说明
    Fluor : Protein ~1:1 Visible absorption maxima 652 Emission maximum 657
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    After product has been reconstituted, product should be stored at 2-8°C in the dark and be used within 3 months. If further dilution of the conjugate is required, use diluted material within one week.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 3 µg/ml.

图片

  • Fluorescence excitation and emission spectra of Allophycocyanin in 100mM sodium phosphate (pH7.2) + 1mM EDTA and 1mM sodium azide. The emission scan was taken with excitation at 630nm. The excitation scan was taken with an emission at 660nm. The curves were normailized to equalize peak heights.

文献

ab99900 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry and your interest in our products.

This secondary antibody has only been tested for Flow cytometry. However, as long as it is compatible with the primary antibody (host species, isotype), it should be fine for immun...

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Vielen Dank für Ihre Anfrage.

Wenn Antikörper mit Biotin gekoppelt werden, wird das meist über freie Anime getan. Das bedeutet das Biotin über den ganzen Antikörper verteilt ist. Die Antikörper werden dann darauf...

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Thank you very much for your feedback.

I am happy to hear that the problem might has been resolved - please keep us updated with the results in FACS as well.

I have looked through your answers, and indeed, with that settings and all...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

In order to understand the problem better and to try to find the origin of the problem, I appreciate if you could confirm the following...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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