山羊Anti-Mouse IgG H&L (HRP) (ab97023)


  • 产品名称山羊Anti-Mouse IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • 经测试应用适用于: ICC, IHC-P, ELISA, WBmore details
  • 偶联物HRP


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明Molar enzyme/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Part of the AbExcel range.
  • 研究领域


Our Abpromise guarantee covers the use of ab97023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000.
WB 1/2000 - 1/20000.

Goat Anti-Mouse IgG H&L (HRP) 图像

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97023, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

Goat Anti-Mouse IgG H&L (HRP) (ab97023)参考文献

This product has been referenced in:
  • Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). Read more (PubMed: 27315119) »
  • Liu QZ  et al. Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Exp Ther Med 12:1651-1656 (2016). ICC/IF . Read more (PubMed: 27588086) »

See all 18 Publications for this product

Product Wall

Application Western blot
Secondary antibody (ab97023) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for Ikb alpha antibody from non abcam brand. Thought the secondary antibody could detect a band at expected 42 kD,
There was a non specific band (at 55 kD was shown by the secondary antibody when used without primary antibody incubation also.
gel: 12%, 200 V for 45 min.
transfer 17 V for 35 min. using Biorad SD.
Blocking 5% BSA.

Abcam user community

Verified customer

提交于 Oct 10 2016

Application Western blot
1. The concentration of Goat Anti-Mouse IgG H&L (HRP) used for this experiment is 1:10000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.

Mr. Lenin Veeraval

Verified customer

提交于 Jun 18 2015

Application Western blot
Used at 1:5000 and works excellently every time.

Abcam user community

Verified customer

提交于 Aug 15 2014

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

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