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mouse whole molecule
Our Abpromise guarantee covers the use of ab6785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)||1/1000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||1/500 - 1/2500. PubMed: 17989178|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.|
|ICC/IF||1/1000 - 1/5000. (PMID16006199)|
|ELISA||1/10000 - 1/50000.|
|WB||1/10000. PubMed: 17217624|
ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"