山羊Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (DyLight® 650) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to小鼠IgG - H&L (DyLight® 650), pre-adsorbed
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details
  • 免疫原

    Other Immunogen Type corresponding to Mouse IgG.

  • 偶联物DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight® 650 replaces DyLight®649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.

Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed 图像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab96882)参考文献

This product has been referenced in:
  • Smith SE  et al. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro. J Virol 87:12957-66 (2013). Flow Cyt . Read more (PubMed: 24067955) »

See 1 Publication for this product

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
ab96882 is used to detect mouse transfferin receptor (red channel), After primary anibody incubation, ab96882 is incubated at room temperature in 2%BSA/PBS for 1h. Confocal imaging showing nice punctate staining on live cells.
Username

Ms. YA ZHOU

Verified customer

提交于 Jan 29 2014

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to investigate this particular case further for you, and al...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"