山羊Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to小鼠IgG - H&L (Cy5 ®), pre-adsorbed
  • 靶标种属Mouse
  • 经测试应用适用于: ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Frmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Mouse IgG, whole molecule (H&L)

  • 偶联物Cy5 ®. Ex: 650nm, Em: 667nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • 共轭说明Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 12.6 moles Cy5 per mole of Goat IgG
  • 克隆多克隆
  • 同种型IgG
  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/50000. PubMed: 18691532
ICC/IF 1/1000 - 1/5000.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed 图像

  • ab2792 (PDI antibody (RL90) - ER marker) staining human U2-OS (bone osteosarcoma) cells by ICC/IF. ab6563 (undiluted) was used as the secondary.

    See Abreview

Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)参考文献

This product has been referenced in:
  • Anastasiades PG  et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun 7:10584 (2016). IHC-FoFr . Read more (PubMed: 26843463) »
  • Sarenac T  et al. Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-ß/Smad pathway of fibrosis in human keratocytes in vitro. Sci Rep 6:34373 (2016). Read more (PubMed: 27687492) »

See all 19 Publications for this product

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
This is a good functioning antibody with a stable conjugate.
The binding is very specific and the luminous intensity of the conjugate is good and also stable. We have used it in the ICC / IF with a 1/1000 Dilution.
Username

Abcam user community

Verified customer

提交于 Oct 06 2015

Thank you for contacting us.

This antibody detects all mouse IgG, so it should work with both your IgG1 and IgG2 antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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Thank you for your inquiry.

I used to perform many ELISPOTs by hand as you with plastic plates. The capture antibody was coated on the plates. The PVDF membrane bottom well plates areused when using a reader to analyze the results. The membra...

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Sorry for the delay in getting back to you.

I can now confirm that the product has been cross adsorbed against the species listed as showing minimal cross reactivity. This includesBovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat,...

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Thank you for contacting us and sorry for the delay in getting back to you in regards to your query.

I have looked into the antibody you are interested in, the Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5 ®), pre-adsorbe...

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Thank you for contacting Abcam.

All of the information regarding purification of the antibodies in our catalogue is available on our website. If we do not specify that Protein A/G was used in the purification process then they were not used t...

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Thank you for your reply.

The information for how each antibody is purified is available on our website. Just go to the webpage for each antibody and the information is there. I have attached a document that shows you where to find that infor...

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Thank you for contacting Abcam.

The antibody ab6563 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities a...

Read More

Thank you very much for your interest in our antibodies. The difference between these two secondary antibodies it that ab6563 is pre-adsorbed against 10 species (chicken, cow, goat, guinea pig, hamster, horse, human, rabbit, rat, sheep), while ab970...

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Thank you for your phone call yesterday and for your questions about ab6563. I have been in touch with the lab, and I was told that small amounts of precipitate are common and should not affect the viability of the antibody. It is possible that the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"