山羊Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to小鼠IgG - H&L (Cy3 ®), pre-adsorbed
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details
  • 偶联物Cy3 ®. Ex: 552nm, Em: 565nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. PubMed: 24270184
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/2000 - 1/4000.

Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed 图像

  • ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.

Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)参考文献

This product has been referenced in:
  • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). ICC/IF . Read more (PubMed: 27611779) »
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC/IF . Read more (PubMed: 25849100) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. The original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. Our results support this claim and the brightness seen in the red channel during image acquisition is considerable. Required exposure times/intensity settings for imaging are, consequently, very low compared to similar fluorophores.
The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (594 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.
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Dr. Sam Nowitzki

Verified customer

提交于 Jul 09 2015

Thank you for your reply.
I'm pleased to hear you would like to participate in the testing discount scheme with the Goat anti-Mouse IgG H&L (Cy3 ®) secondary antibody (ab97035). The discount code is as follows:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"