山羊Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to小鼠IgG - H&L (Biotin), pre-adsorbed
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISAmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/200000. (Chemiluminescent).
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (Primary).

Goat Anti-Mouse IgG H&L (Biotin) preadsorbed (ab97033)参考文献

ab97033 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Used with Timp2 primary antibody ab1828
PAGE: 12%, reducing-denaturing
Blocking with 10% milk overnight
Secondary dilution 1:2000
Detection: ABC system
Band at around 24 kDa
Username

Mrs. Henda Nabli

Verified customer

提交于 Oct 18 2013

Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"