山羊Anti-Mouse IgG H&L (AMCA) (ab47052)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (AMCA)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性This antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins.
  • 经测试应用适用于: ICC/IFmore details
  • 免疫原

    Purified Mouse IgG

  • 偶联物AMCA. Ex: 350nm, Em: 445nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 共轭说明This antibody has been conjugated to AMCA-X, SE [6-((7-Amino 4-methylcoumarin-3-acetyl)amino) hexanoic acid, succinimidyl ester].
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Alternative storage instructions:This product may be aliquoted and frozen at -70°C or below, without glycerol, avoiding repeated freezing and thawing.


    Blue fluorescent with absorption/emission wavelength at 353nm/442nm.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.

Goat Anti-Mouse IgG H&L (AMCA) 图像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:
    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-Ki67, 1:100
    Rabbit anti-laminin alpha 1 (basement marker)
    Secondary antibodies:

    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100
  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

Goat Anti-Mouse IgG H&L (AMCA) (ab47052)参考文献

ab47052 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

Unfortunately, we are not able to offer small sample sizes of our product due to operational reasons. However, we guarantee all our products for TESTED species and applications as listed on the datasheet. The guaran...

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I am very pleased to hear you would like to accept our offer and test ab34766 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please su...

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Thank you for contacting us.

Yes, ab47052 would bind to F(ab')2 since this antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins. The F(ab')2 fragment contains the light chains and part...

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Thank you for your inquiry.

I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be...

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Unfortunately we do not havesecondary antibodies conjugated to Alexa 405 or Pacific Blue.
However, we have the following secondary antibodies, conjugated to AMCA, a blue fluorescence, Excitation/Emission wavele...

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Thank you for calling earlier today.

Below is a list to some secondary antibodies that may be useful to you:

http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-594-pre-adsorbed-ab96881.html
http://...

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Thank you for contacting us.

The information for AMCA is as follows:
Blue fluorescence
Excitation/Emission wavelength = 344 nm / 446 nm

I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: http://www.abcam.com/Easylink

For the information regarding the discount ...

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I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we ...

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Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let...

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