山羊Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)

概述

  • 产品名称山羊Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, Dot blot, WBmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Full length native mouse IgG (purified).

  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
  • 共轭说明Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 克隆多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200 - 1/1000.
ELISA 1/7000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/2000.

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed 图像

  • ab13918 staining RANK in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA/5% normal goat serum/0.1% saponin for 30 minutes at 22°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 9 hours at 4°C. An alkaline phosphatase-conjugated goat anti-mouse IgG H&L (ab7069) (1/300) was used as the secondary antibody.

    See Abreview

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)参考文献

This product has been referenced in:
  • McCarthy JJ  et al. Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development 138:3657-66 (2011). IHC-Fr . Read more (PubMed: 21828094) »
  • Laurentino SS  et al. Regucalcin is broadly expressed in male reproductive tissues and is a new androgen-target gene in mammalian testis. Reproduction 142:447-56 (2011). WB . Read more (PubMed: 21680783) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-CD68 (mouse) with Goat anti Mouse ALP (on human artery)

-Deparaffinize

-heat in citrate buffer 20min at 96°C

-H2O2 for 10min at room temperature

-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature

-incubate anti CD68 at 1/100 in PBS BSA Overnight at 4°C

-incubate with goat anti mouse ALP (1/300) for 45min at 37°C

-incubate slides in alkaline phosphatase substrate

-counterstain with hematoxylin, dehydrate and mount
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Abcam user community

Verified customer

提交于 Mar 13 2014

Thank you for your inquiry. I've included the link to the secondary antibody that we talked about on the phone.  This would work if your other antibody is raised in a mouse.  I've also included a link to some of our substrate kits. http://www.abc...

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Thank you for your enquiry. As you mentioned in your e-mail, we currently have two Goat anti-mouse AP conjugated secondary antisera: ab7069 and ab6790. ab7069 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"