山羊Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

概述

  • 产品名称山羊Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性Binds IgG; does not bind other Igs.
  • 经测试应用适用于: WB, ELISA, IHC-P, Dot blotmore details
  • 最小交叉反应
    Cow, Horse, Human more details
  • 免疫原

    Purified mouse IgG, Fc fragment

  • 阳性对照Human tonsil tissue
  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8.00
    Preservative: 0.25% Sodium azide
    Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media
  • 共轭说明Goat anti-mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
Dot blot
  • 应用说明Dot: 1/30000 - 1/100000.
    ELISA: 1/30000.
    IHC-P: 1/40.
    WB: 1/160000 when using chemiluminescent detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)参考文献

    ab49724 has not yet been referenced specifically in any publications.

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