山羊Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

概述

  • 产品名称
    山羊Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 特异性
    Binds IgG; does not bind other Igs.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, Dot blotmore details
  • 最小交叉反应

    Cow, Horse, Human more details
  • 免疫原

    Purified mouse IgG, Fc fragment

  • 阳性对照
    Human tonsil tissue
  • 偶联物
    Alkaline Phosphatase

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 8.00
    Preservative: 0.25% Sodium azide
    Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media
  • 共轭说明
    Goat anti-mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
Dot blot
  • 应用说明
    Dot: 1/30000 - 1/100000.
    ELISA: 1/30000.
    IHC-P: 1/40.
    WB: 1/160000 when using chemiluminescent detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    ab49724 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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