Goats were immunized with highly purified pooled normal IgM.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
0.01M Tris-0.14M sodium chloride pH 7-7.2
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Immunogen affinity purified
After several immunizations with complete Freunds adjuvant the resulting antiserum was solid phase absorbed to make it mu chain specific.
The resulting anti mu chain antibodies were immunoaffinity purified off an IgM immunoabsorbant.