山羊Anti-Human IgM H&L (FITC) (ab8497)

概述

  • 产品名称山羊Anti-Human IgM H&L (FITC)
    参阅全部 IgM 二抗
  • 靶标种属Human
  • 特异性Antibodies were raised in goat to human IgM
  • 经测试应用适用于: ICC/IFmore details
  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound.
  • 共轭说明The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • 克隆多克隆
  • 轻链类型unknown
  • 常规说明F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • Goat Anti-Human IgM H&L (FITC) (ab8497)参考文献

    ab8497 has not yet been referenced specifically in any publications.

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