山羊抗人IgG H&L (HRP)预吸附二抗(ab97175)

概述

  • 产品名称
    山羊抗人IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to人IgG - H&L (HRP),预吸附二抗
  • 宿主
    Goat
  • 靶标种属
    Human
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
  • 经测试应用
    适用于: ICC, IHC-P, ELISA, WBmore details
  • 最小交叉反应

    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
  • 免疫原

    Other Immunogen Type corresponding to Human IgG.

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明
    Molar enzyme/ antibody protein ratio is 4:1
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. ELISA-Primary
WB 1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000

文献

This product has been referenced in:
  • Mishra N  et al. Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay. MBio 9:N/A (2018). Read more (PubMed: 29511073) »
  • Torres M  et al. Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFa production in CHO cells. PLoS One 13:e0194510 (2018). Read more (PubMed: 29566086) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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