山羊抗人IgG H&L (HRP)预吸附二抗(ab97175)
Key features and details
- Goat Anti-Human IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
山羊抗人IgG H&L (HRP)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Human -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
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经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
最小交叉反应
Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details -
免疫原
Other Immunogen Type corresponding to Human IgG.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97175于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. ELISA-Primary
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WB |
1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000
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说明 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. ELISA-Primary |
WB
1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000 |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab97175 被引用在 11 文献中.
- Lenhart-Pendergrass PM et al. Deficient Complement Opsonization Impairs Mycobacterium avium Killing by Neutrophils in Cystic Fibrosis. Microbiol Spectr 11:e0327922 (2023). PubMed: 36651756
- Rafat N et al. Enhanced Enzymatically Amplified Metallization on Nanostructured Surfaces for Multiplexed Point-of-Care Electrical Detection of COVID-19 Biomarkers. Small 18:e2203309 (2022). PubMed: 36036173
- Nick JA et al. Host and pathogen response to bacteriophage engineered against Mycobacterium abscessus lung infection. Cell 185:1860-1874.e12 (2022). PubMed: 35568033
- Malcolm KC et al. Specificity of Immunoglobulin Response to Nontuberculous Mycobacteria Infection in People with Cystic Fibrosis. Microbiol Spectr 10:e0187422 (2022). PubMed: 35863022
- Harpaz D et al. Enhanced Colorimetric Signal for Accurate Signal Detection in Paper-Based Biosensors. Diagnostics (Basel) 10:N/A (2020). PubMed: 31936174