山羊Anti-Human IgG H&L (HRP) preadsorbed (ab7153)

概述

  • 产品名称山羊Anti-Human IgG H&L (HRP) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • 免疫原

    Full length native human IgG (purified).

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Horseradish Peroxidase (HRP)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
ELISA 1/7000 - 1/200000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Goat Anti-Human IgG H&L (HRP) preadsorbed (ab7153)参考文献

ab7153 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.
Both of the secondary antibodies chosen (ab102429 and ab7153) should be able to detect the primary antibody (human IgG3) in the ELISA the customer is hoping to perform.
I hope this ...

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Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. ...

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Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"