山羊Anti-Human IgG H&L (HRP) (ab81202)

概述

  • 产品名称山羊Anti-Human IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 特异性No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    Human IgG.

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.50
    Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • 共轭说明The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100.
ELISA 1/1000.

Goat Anti-Human IgG H&L (HRP) (ab81202)参考文献

ab81202 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your questions. Ab81202 was raised against human IgG, and since ab81202 is polyclonal, I expect it to react with both the heavy and light chains. I do not think that this has been specifically tested. Ab81202 has...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"