山羊Anti-Human IgA H&L (FITC) (ab8500)

概述

  • 产品名称山羊Anti-Human IgA H&L (FITC)
    参阅全部 IgA 二抗
  • 靶标种属Human
  • 特异性Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • 经测试应用适用于: ICC/IFmore details
  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • 共轭说明The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • 克隆多克隆
  • 常规说明F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgA.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • Goat Anti-Human IgA H&L (FITC) (ab8500)参考文献

    ab8500 has not yet been referenced specifically in any publications.

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