特异性Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
存储溶液50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
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纯度Immunogen affinity purified
纯化说明From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG.
Specific antibodies were absorpted by incubation with Sepharose-bound human IgA.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human IgA a minimum of 65% protein bound.
共轭说明The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
常规说明F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgA.