山羊Anti-Human IgA H&L (ab8499)

概述

  • 产品名称
    山羊Anti-Human IgA H&L
    参阅全部 IgA 二抗
  • 靶标种属
    Human
  • 特异性
    Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • 经测试应用
    适用于: ELISA, Dot blotmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • 克隆
    多克隆
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Dot blot
  • 应用说明
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • Goat Anti-Human IgA H&L (ab8499)参考文献

    ab8499 has not yet been referenced specifically in any publications.

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