山羊抗人IgA alpha chain (DyLight® 650) (ab96998)
Key features and details
- Goat Anti-Human IgA alpha chain (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊抗人IgA alpha chain (DyLight® 650)
参阅全部 IgA 二抗 -
宿主
Goat -
靶标种属
Human -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cytmore details -
免疫原
Other Immunogen Type corresponding to Human IgA.
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偶联物
DyLight® 650. Ex: 654nm, Em: 673nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 650 replaces DyLight® 649. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96998于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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说明 |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96998 被引用在 4 文献中.
- Wilson NG et al. The gut microbiota of people with asthma influences lung inflammation in gnotobiotic mice. iScience 26:105991 (2023). PubMed: 36824270
- Pape C et al. Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera. Bioessays 43:e2000257 (2021). PubMed: 33377226
- Gruber CN et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell 183:982-995.e14 (2020). PubMed: 32991843
- Planer JD et al. Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice. Nature 534:263-6 (2016). PubMed: 27279225