山羊F(ab')2 Anti-大鼠IgG - H&L (HRP), pre-adsorbed (ab6120)

概述

  • 产品名称
    山羊F(ab')2 Anti-大鼠IgG - H&L (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Rat
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • 经测试应用
    适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 最小交叉反应

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Rat IgG whole molecule

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
WB
ELISA
Electron Microscopy
IHC
  • 应用说明
    Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/16000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product.

    Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed (ab6120)参考文献

    This product has been referenced in:
    • Singh MK  et al. MicroRNA-processing Enzyme Dicer Is Required in Epicardium for Coronary Vasculature Development. J Biol Chem 286:41036-45 (2011). IHC-Fr . Read more (PubMed: 21969379) »
    • Leng X  et al. Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats. Cell Stress Chaperones 15:947-58 (2010). ELISA . Read more (PubMed: 20607472) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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