山羊F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed (ab6115)

概述

  • 产品名称山羊F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • 最小交叉反应
    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Rat IgG whole molecule

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.2 moles FITC per mole of Goat IgG F(ab’)2
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000. PubMed:17173070
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

Goat F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed (ab6115)参考文献

This product has been referenced in:
  • Louboutin JP  et al. Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes. Neurobiol Dis 38:313-25 (2010). Read more (PubMed: 20219678) »
  • Bouvard C  et al. alpha6-integrin subunit plays a major role in the proangiogenic properties of endothelial progenitor cells. Arterioscler Thromb Vasc Biol 30:1569-75 (2010). Read more (PubMed: 20508204) »

See all 2 Publications for this product

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