Our Abpromise guarantee covers the use of
ab6119
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 |
Ab评论 |
说明 |
ICC/IF |
|
|
Dot blot |
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WB |
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ELISA |
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IHC |
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Immunomicroscopy |
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应用说明
Dot: Use at an assay dependent dilution.
ELISA: 1/26000 - 1/142000.
IHC: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/26000 to 1/142000 of the reconstitution concentration is suggested for this product.
Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.