山羊F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed (ab6257)

概述

  • 产品名称山羊F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Electron Microscopymore details
  • 最小交叉反应
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(c) whole molecule

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
    (Warning: use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
WB
ELISA
IHC
Electron Microscopy
  • 应用说明Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/20000 - 1/200000.
    IHC: 1/500 - 1/5000.
    WB: 1/20000 - 1/200000.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections.

    Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed (ab6257)参考文献

    This product has been referenced in:
    • Gomez AM  et al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA . Read more (PubMed: 21239719) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"