山羊F(ab')2抗大鼠IgG - (Fab)'2 (TRITC ),预吸附二抗(ab6270)

概述

  • 产品名称
    山羊F(ab')2抗大鼠IgG - (Fab)'2 (TRITC ),预吸附二抗
    参阅全部 IgG 二抗
  • 宿主
    Goat
  • 靶标种属
    Rat
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • 经测试应用
    适用于: ICC/IF, ELISA, Flow Cyt, Immunomicroscopymore details
  • 最小交叉反应

    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(ab’)2 fragment

  • 偶联物
    TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.2 moles TRITC per mole of Goat IgG F(ab’)2
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
ELISA
Flow Cyt
Immunomicroscopy
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    This product has been referenced in:
    • Liu Q  et al. C1q/TNF-related protein 9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP-dependent kinase. J Cell Mol Med 21:2823-2836 (2017). Read more (PubMed: 28524645) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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