山羊F(ab')2抗大鼠IgG - (Fab)'2 (TRITC ),预吸附二抗(ab6270)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称山羊F(ab')2抗大鼠IgG - (Fab)'2 (TRITC ),预吸附二抗
参阅全部 IgG 二抗 -
宿主Goat
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靶标种属Rat
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特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
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经测试应用适用于: ICC/IF, ELISA, Flow Cyt, Immunomicroscopymore details
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最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab’)2 fragment
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偶联物TRITC. Ex: 547nm, Em: 572nm
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading... -
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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共轭说明Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.2 moles TRITC per mole of Goat IgG F(ab’)2
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克隆多克隆
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab6270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| Flow Cyt | ||
| Immunomicroscopy |
应用说明
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
文献
This product has been referenced in:
- Liu Q et al. C1q/TNF-related protein 9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating AMP-dependent kinase. J Cell Mol Med 21:2823-2836 (2017). Read more (PubMed: 28524645) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"