山羊F(ab')2 Anti-Rat IgG - (Fab)'2 (PE), pre-adsorbed (ab6519)

概述

  • 产品名称山羊F(ab')2 Anti-Rat IgG - (Fab)'2 (PE), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • 经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(ab’)2 fragment

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.6
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.5
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Immunomicroscopy
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (PE), pre-adsorbed (ab6519)参考文献

    ab6519 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab6519.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"