山羊F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517)

概述

  • 产品名称山羊F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 最小交叉反应
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(ab’)2 fragment

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.20
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.16% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed (ab6517)参考文献

This product has been referenced in:
  • Motomura E  et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . Read more (PubMed: 25330272) »
  • Wang SH  et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). Read more (PubMed: 23363740) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"