山羊F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)

概述

  • 产品名称山羊F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • 最小交叉反应
    Cow, Horse, Human more details
  • 免疫原

    Rat IgG F(ab')2 fragment

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/400000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)参考文献

ab6275 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"