山羊F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab6013)

概述

  • 产品名称
    山羊F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • 经测试应用
    适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/115000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab6013)参考文献

This product has been referenced in:
  • Chou MH  et al. Effects of hepatocyte CD14 upregulation during cholestasis on endotoxin sensitivity. PLoS One 7:e34903 (2012). WB . Read more (PubMed: 22511970) »
  • Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). Read more (PubMed: 21362625) »

See all 2 Publications for this product

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to gen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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