山羊F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

概述

  • 产品名称山羊F(ab')2 Anti-Rabbit IgG H&L (10nm Gold)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: Electron Microscopymore details
  • 免疫原

    Rabbit IgG whole molecule.

  • 偶联物Gold 10nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy
  • 应用说明Electron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)参考文献

    This product has been referenced in:
    • Wu JL  et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015). Read more (PubMed: 25993499) »

    See 1 Publication for this product

    Product Wall

    The F(ab')2 concentration for ab39601, Goat F(ab')2 Anti-Rabbit IgG 10nm gold conjugate, is 10 microgram/ml. The gold particle concentration of the 10nm gold conjugate is approximately 5x10e12 particles per ml.

    Application Immunohistochemistry
    Human skin containing 3-4 hair follicles was fixed in glutaraldehyde/paraformaldehyde, embedded in London resin, sectioned at 75nm, subjected to a protocol of potassium periodate antigen retrieval and treated with 2ug/ml of a rabbit anti- KRTAP5 antibody followed by Abcam Goat anti-rabbit IgG 10nm gold. at a dilution of 1/25 The localisation was excellent and specific to the keratin in the cuticle and cortex layers of the developing cuticle and cortex. The binding is to the exocuticle and also the darker layer at the edges of the cuticle cells, the a-layer Reaction also occurs with the cortex (upper right) because the KAPs in the cortex share amino acid sequences. Control (no antibody) was negative.
    GE Rogers
    Biochemistry
    The University of Adelaide' South Australia
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"