山羊F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485)

概述

  • 产品名称山羊F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: IHC-P, ELISA, WB, ICCmore details
  • 最小交叉反应
    Human, Mouse, Rat more details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. (Colorimetric: /2,000-1/20,000 dilution; Chemiluminescent: 1/5,000-1/25,000 dilution).
ICC Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rabbit IgG - Fc (HRP), pre-adsorbed (ab98485)参考文献

ab98485 has not yet been referenced specifically in any publications.

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Abreviews
Application ELISA
HEK293 cells engineered to stably overexpress the human Leptin Receptor (hLR) were stimulated with increasing doses of recombinant human Leptin (rhLeptin). The cells were fixed using formaldehyde, permeabilized, quenched, and blocked using proprietary buffers and then incubated overnight at 4°C with a rabbit anti-pSTAT3 (Y705) antibody (abcam, ab76315, 1/500 dilution). Subsequently, the cells were washed and incubated 30 min at room temperature with a HRP-conjugated goat anti-rabbit F(ab')2 (abcam, ab98485). The cells were washed and TMB substrate was added. TMB conversion was stopped and absorbance was read at 450 nm. The cells were washed and stained using JanusGreen. JanusGreen was eluted and absorbance was read at 630 nm.
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提交于 Oct 05 2016

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