山羊F(ab')2 Anti-Rabbit IgG Fc (Biotin) (ab6021)

概述

  • 产品名称山羊F(ab')2 Anti-Rabbit IgG Fc (Biotin)
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab’)2.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IPmore details
  • 免疫原

    Rabbit IgG F(c) fragment

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab’)2 molecule
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin) (ab6021)参考文献

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If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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