山羊F(ab')2抗兔IgG - (Fab')2,预吸附二抗(ab102288)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
概述
-
产品名称
山羊F(ab')2抗兔IgG - (Fab')2,预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-P, ICC, ELISA, WBmore details -
最小交叉反应
Human, Mouse, Rat more details
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/200 - 1/2000.
|
|
ICC |
1/200 - 1/2000.
|
|
ELISA |
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
|
|
WB |
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).
|
说明 |
---|
IHC-P
1/200 - 1/2000. |
ICC
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000). |
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000). |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (1)
ab102288 被引用在 1 文献中.
- Li Y et al. Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus. mBio 13:e0035822 (2022). PubMed: 36102516