山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed (ab6108)

概述

  • 产品名称
    山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • 最小交叉反应

    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG F(ab’)2 fragment

  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Goat IgG F(ab’)2
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    This product has been referenced in:
    • Bergsbaken T  et al. Local Inflammatory Cues Regulate Differentiation and Persistence of CD8(+) Tissue-Resident Memory T Cells. Cell Rep 19:114-124 (2017). Read more (PubMed: 28380351) »
    • Krelin Y  et al. Association between tumorigenic potential and the fate of cancer cells in a syngeneic melanoma model. PLoS One 8:e62124 (2013). Flow Cyt ; Rabbit . Read more (PubMed: 23626777) »

    See all 2 Publications for this product

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    You are very welcome. Please feel to contact us if you have any further questions!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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