山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed (ab6108)

概述

  • 产品名称山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • 最小交叉反应
    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG F(ab’)2 fragment

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Goat IgG F(ab’)2
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed (ab6108)参考文献

    This product has been referenced in:
    • Krelin Y  et al. Association between tumorigenic potential and the fate of cancer cells in a syngeneic melanoma model. PLoS One 8:e62124 (2013). Flow Cyt ; Rabbit . Read more (PubMed: 23626777) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"