山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (AP), pre-adsorbed (ab6113)

概述

  • 产品名称山羊F(ab')2 Anti-Rabbit IgG - (Fab)'2 (AP), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • 最小交叉反应
    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG F(ab')2 fragment

  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
WB
ELISA
IHC
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/500 - 1/2000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product.

    Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (AP), pre-adsorbed (ab6113)参考文献

    This product has been referenced in:
    • Sekine-Suzuki E  et al. Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells. Biochem Biophys Res Commun 377:341-5 (2008). WB . Read more (PubMed: 18854174) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for your inquiry. Reviewing the datasheets for these antibody I am confident that a single ELISPOT should give good results with this combination of antibodies. But unfortunately, these antibodies have not been tested in...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"