山羊F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgM mu chain (TRITC)
    参阅全部 IgM 二抗
  • 描述
    山羊F(ab')2多克隆抗体二抗to小鼠IgM - mu chain (TRITC )
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • 经测试应用适用于: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 免疫原

    Mouse IgM mu chain

  • 偶联物TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG F(ab’)2
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.

Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)参考文献

This product has been referenced in:
  • Ishikawa M  et al. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci 55:8531-41 (2014). ICC/IF ; Rat . Read more (PubMed: 25406290) »

See 1 Publication for this product

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