山羊F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin) (ab5932)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin)
    参阅全部 IgM 二抗
  • 靶标种属Mouse
  • 特异性Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins.
  • 经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details
  • 免疫原

    Mouse IgM mu heavy chain

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Goat F(ab')2 Anti-Mouse IgM mu chain (Phycoerythrin) (ab5932)参考文献

This product has been referenced in:
  • Lappalainen J  et al. A protocol for generating high numbers of mature and functional human mast cells from peripheral blood. Clin Exp Allergy 37:1404-14 (2007). Flow Cyt ; Mouse . Read more (PubMed: 17845422) »

See 1 Publication for this product

Product Wall

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cr...

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