山羊F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)

概述

  • 产品名称
    山羊F(ab')2 Anti-Mouse IgM mu chain (HRP)
    参阅全部 IgM 二抗
  • 靶标种属
    Mouse
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 免疫原

    Mouse IgM mu chain

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.3% Potassium phosphate
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
Dot blot 1/5000 - 1/100000.
WB 1/2000 - 1/10000.
ELISA 1/90000.
Electron Microscopy Use at an assay dependent dilution.
IHC 1/500 - 1/5000.

Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)参考文献

This product has been referenced in:
  • Hasham MG  et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . Read more (PubMed: 28250051) »
  • Rieder LE  et al. Dynamic response of RNA editing to temperature in Drosophila. BMC Biol 13:1 (2015). Read more (PubMed: 25555396) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry. Well done on getting your western blot to work. I have read your comments and have examined your blot images. I would like ot take each of your antibodies one at a time: ab3568 - Cytochrome P450 1A1 antibody (ab3...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册