山羊F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

概述

  • 产品名称山羊F(ab')2 Anti-Mouse IgM mu chain (FITC)
    参阅全部 IgM 二抗
  • 描述
    山羊F(ab')2多克隆抗体二抗to小鼠IgM - mu chain (FITC)
  • 靶标种属Mouse
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • 经测试应用适用于: ICC/IF, Flow Cyt, ELISAmore details
  • 免疫原

    Mouse IgM mu chain

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) 图像

  • Ab5926 was used at dilution 1/100 with the primary antibody ab2784 in Flow Cyt. See the review on ab2784.

Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)参考文献

This product has been referenced in:
  • Zhong Y  et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . Read more (PubMed: 20846785) »
  • Wang K  et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Human, Rhesus monkey . Read more (PubMed: 20025521) »

See all 2 Publications for this product

Product Wall

Hello and thank you for your patience is trying to troublshooting your Flow Cytometry experiment. I have noticed the secondary you are using is specific to the mu chain of mouse IgM. The primary antibodies are to total IgM, so this is likely the reason...

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Thank you for contacting us.

ab5926 shows less than 1% cross reactivity against other mouse heavy or light chain isotypes in ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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Thank you for your enquiry. Ab5926 has not been tested for cross-reactivity with human IgM, just mouse IgM. We do have several human IgM secondary antibodies (for example ab8506), but unfortunately not a Fab-fragment secondary antibody to human IgM....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"