山羊F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

概述

  • 产品名称
    山羊F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    参阅全部 IgM 二抗
  • 靶标种属
    Mouse
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details
  • 免疫原

    Mouse IgM mu heavy chain

  • 偶联物
    Biotin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明
    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
IHC 1/500 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/200 - 1/1000.

图片

  • Ab5929 was used with the primary antibody ab8226 in WB. See Abreview on ab8226.

文献

This product has been referenced in:
  • Grewal N  et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . Read more (PubMed: 25502628) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positiv...

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We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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