山羊F(ab')2抗小鼠IgM mu chain (Biotin) (ab5929)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (Biotin)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
经测试应用
适用于: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details -
免疫原
Mouse IgM mu heavy chain
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500 - 1/2500.
|
|
ELISA |
1/10000 - 1/50000.
|
|
WB |
1/1000 - 1/5000.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
IHC |
1/500 - 1/5000.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Flow Cyt |
1/200 - 1/1000.
|
说明 |
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ICC/IF
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
WB
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/200 - 1/1000. |
图片
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ab5929 was used as the secondary antibody in WB.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab5929 被引用在 3 文献中.
- Liu HY et al. Leveraging the Treg-intrinsic CTLA4-PKC? signaling pathway for cancer immunotherapy. J Immunother Cancer 9:N/A (2021). PubMed: 34588224
- Hong R et al. Distinct roles of BCNP1 in B-cell development and activation. Int Immunol 32:17-26 (2020). PubMed: 31412363
- Grewal N et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628